STUDY FINDS CHANGES IN BLOOD NOT HEART MAY UNDERLIE CARDIAC THROMBOSIS IN COVID-19 PATIENTS

A team of researchers examined autopsy tissue samples of hearts from patients who died early in the COVID-19 pandemic.

Frequent and extensive blood clots (thromboses) within heart vessels were found as anticipated, but the type of changes in the endothelial cells lining the heart that are typically observed in thromboses was absent. Instead, data indicated the likely culprit to be hypercoagulability of the blood caused by activated neutrophils, a type of white blood cell.

“My laboratory has a long history of defining endothelial cell alterations that produce pathologies, including thrombosis, and we expected to confirm the widely held assumption that local endothelial cell alterations were responsible for thrombosis of the cardiac vessels in COVID-19 patients,” explained lead investigator Jordan S. Pober, MD, PhD, Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.

“Instead, we found that the cardiac thrombi contained neutrophils that expressed changes known to promote coagulation, including changes that are associated with cell death and inflammation,” he added.

Hospitalized patients with SARS-CoV-2 infection have an increased risk of developing a myocardial injury. However, numerous studies have rarely detected viral protein or RNA within the hearts of patients who died from COVID-19, despite evidence of abundant virus presence in the lungs of the same patients. Thrombosis of micro and macro coronary vessels has most consistently characterized the hearts of individuals who succumbed to COVID-19, but the underlying cause remains unknown.

Dr Pober and his colleagues examined heart tissue from seven autopsies of COVID-19 patients performed early in the pandemic, before anticoagulation treatment was commonly administered, and compared these specimens to autopsy tissue from 12 COVID-19-negative controls, with and without heart disease, using multiparameter fluorescence microscopy to analyze the composition of the thrombosed vessels. All patients in the COVID-19 group had severe pneumonia. One patient experienced a sudden cardiac arrest outside of the hospital, two patients developed sepsis, and one patient had recurrent acute leukaemia with thrombocytopenia. The COVID-19-negative controls included six patients with pre-existing cardiac disease. Thrombosis was the most common pathological finding in the COVID-19 group with a greatly elevated frequency of the microthrombi and a total number of macrothrombi compared to the COVID-19-negative controls. Despite the widespread evidence of thrombosis, no evidence of myocyte death or acute inflammation typically associated with myocardial infarction was detected in the COVID-19 group.

The vessels of the heart were examined for signs of endothelial cell injury, which can promote thrombosis through the release of microparticles containing procoagulative tissue factor, or by endothelial cell sloughing that can expose platelet-activating collagen. The investigators failed to find such endothelial changes at sites of thrombosis. Instead, they saw that the cardiac thrombi in four of the six COVID-19 patients contained neutrophils that expressed procoagulant changes in the blood, such as citrullination of histones associated with the formation of neutrophil extracellular traps(NETs). Some images suggested NETS that appeared to be directly associated with platelets. Neutrophil-rich macrothrombi composed of 30 per cent or more neutrophils were common in the COVID-19 group but not in control tissue specimens.

Dr Pober commented, “Our data challenge the view that alterations in the heart vessel wall are the primary cause of COVID-19 cardiac thrombosis. Current treatments of severe COVID-19 include anticoagulation, but the best strategy is still not clear. In light of our findings, reducing neutrophil responses could be an important target for therapeutic intervention. This and many other advances in the understanding of disease continue to be provided by autopsies, and I am grateful to the pathologists who performed them for this study at both Brigham and Women’s Hospital and Yale.”

Peter Libby, MD, a cardiologist and vascular biologist at Boston’s Brigham and Women’s Hospital and the Harvard Medical School, a long-time collaborator of Dr Pober’s, stated: “For several years we have studied neutrophils and their prothrombotic products known as NETs in the context of clots that form in the larger coronary arteries.